Article
Intracellular carbonic anhydrase is essential to photosynthesis in Chlamydomonas reinhardtii at atmospheric levels of CO2. Demonstration via genomic complementation of the high-CO2-requiring mutant ca-1.
Plant physiology - 1 May 1997
Funke R P, Kovar J L, Weeks D P
Abstract excerpt
Genomic complementation of the high-CO2-requiring mutant ca-1-12-1C of Chlamydomonas reinhardtii was achieved by transformation with DNA pools from an indexed cosmid library of wild-type genomic DNA. Transformation of mutant cells with cosmid DNA from two microtiter plates in the library produced...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Carbon Dioxide
- Carbonic Anhydrases
- Chlamydomonas reinhardtii
- Cloning, Molecular
- Cosmids
- DNA, Protozoan
- Genes, Protozoan
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Photosynthesis
- Transformation, Genetic