Article
Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear protein, involved in control of higher order chromosome structure and gene expression.
The Journal of biological chemistry - 4 Mar 1994
Nishi K, Yoshida M, Fujiwara D, Nishikawa M, Horinouchi S, Beppu T
Abstract excerpt
The molecular action of leptomycin B (LMB), an agent inducing arrest of the eukaryotic cell cycle at G1 and G2 phases, was investigated by analyzing an LMB resistance gene of Schizosaccharomyces pombe. A genomic library of an LMB-resistant mutant was screened for LMB resistance, and a DNA fragmen...
Topics
- Alkaloids
- Alleles
- Antifungal Agents
- Chromosomes, Fungal
- Cloning, Molecular
- Cycloheximide
- DNA, Fungal
- Drug Resistance, Microbial
- Fatty Acids, Unsaturated
- Fungal Proteins
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Karyopherins
- Nuclear Proteins
- Open Reading Frames
- Point Mutation
- Protein Kinase C
- Receptors, Cytoplasmic and Nuclear