Importance of the proline-rich region following signal-anchor sequence in the formation of correct conformation of microsomal cytochrome P-450s.

Abstract excerpt

A proline-rich region is present following the signal-anchor sequence in the amino-terminal portion of all known microsomal cytochrome P-450s. To assess the functional significance of the proline residues in this region, we systematically altered these residues of cytochrome P450(M1) (P450 2C11); one, two, and three proline residues out of the five in the region were exchanged for alanine residues. The wild-type...

Topics

• Amino Acid Sequence
• Animals
• Aryl Hydrocarbon Hydroxylases
• Base Sequence
• Cytochrome P-450 Enzyme System
• Electrophoresis, Polyacrylamide Gel
• Hydroxylation
• Microsomes, Liver
• Molecular Sequence Data
• Mutation