Article
Reversion of a simian virus 40 enhancer mutant depends on the growth conditions.
Gene - 25 Feb 1994
Martínez-Balbás M A
Abstract excerpt
The simian virus 40 (SV40) enhancer contains three 8-bp purine-pyrimidine (R:Y) alternating sequences (Z-motifs) which are known to adopt the left-handed Z-DNA conformation in vitro. Mutations at these three Z-motifs seriously impair enhancer function. Reversion of one of these mutants (dpm12) is studied in this paper. The results indicate that, depending on growth conditions, recovery of the enhancer function is...
Topics
- Base Sequence
- Cells, Cultured
- Culture Media
- Enhancer Elements, Genetic
- Genes, Viral
- Molecular Sequence Data
- Multigene Family
- Mutation
- Simian virus 40
- Transfection