Article
Perforin and granzyme B expression is associated with severe acute rejection. Evidence for in situ localization in alveolar lymphocytes of lung-transplanted patients.
Transplantation - 1 Feb 1994
Clément M V, Legros-Maïda S, Israël-Biet D, Carnot F, Soulié A, Reynaud P, Guillet J, Gandjbakch I, Sasportes M
Abstract excerpt
To evaluate rejection episodes in lung-transplanted patients, we analyzed 31 bronchoalveolar lavage specimens for lymphocyte levels and lymphocyte expression of two intracytoplasmic activation markers, perforin, the pore-forming lytic protein, and granzyme B, a member of the serine esterase family. Using anti-human granzyme B and perforin mAbs, we show that their expression in alveolar lymphocytes is correlated...
Topics
- Biopsy
- Bronchi
- Bronchoalveolar Lavage Fluid
- Cell Count
- Graft Rejection
- Granzymes
- Humans
- Immunohistochemistry
- Lung Transplantation
- Lymphocytes
- Membrane Glycoproteins