Article
Classification of mutations at the HLA-A locus by use of the polymerase chain reaction.
Environmental and molecular mutagenesis - 1 Jan 1993
Joseph G, Grist S, Firgaira F, Turner D, Morley A
Abstract excerpt
We investigated whether the polymerase chain reaction (PCR) could be used to determine the mechanism of mutation in lymphocyte clones mutated at the HLA-A locus. Three polymorphisms, at Factor XIIIA, D6S109, and intron 3 of the HLA-A gene, were used to study a series of clones previously characte...
Topics
- Base Sequence
- Blotting, Southern
- Chromosomes, Human, Pair 6
- Cloning, Molecular
- DNA Mutational Analysis
- DNA Primers
- Gene Conversion
- Gene Deletion
- Genes, MHC Class I
- HLA-A Antigens
- Heterozygote
- Humans
- Lymphocytes
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length