Article
CD45-negative mutants of a rat natural killer cell line fail to lyse tumor target cells.
Journal of immunology (Baltimore, Md. : 1950) - 1 Oct 1993
Bell G M, Dethloff G M, Imboden J B
Abstract excerpt
To examine the role of CD45 in NK cell activation, we isolated three mutants and one variant of a rat NK cell line, RNK-16. Each of these lacked cell-surface expression of CD45 and did not have detectable transcripts for CD45 on Northern blot analysis. The CD45-negative cells expressed CD2, CD53, and NKR-P1, but mAb-induced perturbations of these molecules did not induce protein tyrosine phosphorylations and...
Topics
- Animals
- Antigens, CD
- Antigens, Differentiation, T-Lymphocyte
- CD2 Antigens
- Cell Line
- Cytotoxicity, Immunologic
- Killer Cells, Natural
- Leukocyte Common Antigens
- Mutation
- Protein-Tyrosine Kinases
- Rats