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Article

Selection of Chinese hamster ovary cells (CHO-K1) with reduced glutamate and aspartate uptake.

Somatic cell and molecular genetics - 1 May 1993

Ash J F, Igo R P, Morgan M, Grey A

Abstract excerpt

Transport of L-[3H]glutamate into Chinese hamster ovary cells (CHO-K1) was characterized and the results used to design a tritium suicide selection for cells with transport defects. Replicas of surviving colonies on polyester cloth disks were screened by autofluorography for reduced uptake and two mutant clones, Ed-A1 and Ed-B8, were obtained. Uptake of glutamate through a sodium-dependent system in both mutants...

Topics

Animals
Aspartic Acid
Biological Transport
CHO Cells
Cell Death
Cell Separation
Cricetinae
Glutamates
Glutamic Acid
Kinetics
Mutation

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Identifiers

PMID: 8101392
DOI: 10.1007/BF01233071

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8101392-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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