Article
A mutant Synechococcus gene encoding glutamate 1-semialdehyde aminotransferase confers gabaculine resistance when expressed in tobacco plastids.
Plant cell reports - 1 Dec 2015
Bellucci Michele, De Marchis Francesca, Ferradini Nicoletta, Pompa Andrea, Veronesi Fabio, Rosellini Daniele
Abstract excerpt
KEY MESSAGE: A mutant glutamate 1-semialdehyde aminotransferase gene from the Synechococcus , inserted into tobacco plastid DNA by means of particle bombardment and antibiotic selection, conferred gabaculine resistance allowing to attain homoplasmy. Many plant species are recalcitrant to plastid genome transformation. New selections systems may help to overcome this limitation and to extend the application of...
Topics
- Bacterial Proteins
- Cyclohexanecarboxylic Acids
- Enzyme Inhibitors
- Genetic Markers
- Intramolecular Transferases
- Medicago sativa
- Mutation
- Photosynthesis
- Plants, Genetically Modified
- Plastids
- Synechococcus
- Nicotiana
- Triticum