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Article

Selection of a lysine-resistant CHO-K1 mutant with reduced amino acid transport through multiple systems.

Somatic cell and molecular genetics - 1 Jul 1993

DeBusk W E, Ash J F

Abstract excerpt

High levels of L-lysine were used to select for resistant variants of Chinese hamster ovary (CHO-K1) cells. Surviving colonies were screened for altered lysine transport and two with reduced uptake were picked. Clone CH-Kr, derived from the more severely affected colony, was analyzed in detail. In starved cells the Vmax of lysine uptake in CH-Kr was half that of CHO while Km was unaltered. The intracellular pool...

Topics

Amino Acids
Animals
Biological Transport
CHO Cells
Cell Division
Cloning, Molecular
Cricetinae
Drug Resistance
Kinetics
Lysine
Mutation

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PMID: 8211377
DOI: 10.1007/BF01232746

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8211377-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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