Article
Determination of pig apolipoprotein B genotype by gene amplification and restriction fragment length polymorphism analysis.
Animal genetics - 1 Apr 1993
Kaiser M E, Nevin D N, Sturley S L, Purtell C, Attie A D
Abstract excerpt
Sequence differences within the pig apoB gene can be used to identify rapidly four of eight known pig apoB alleles, designated LPB1-LBP8. We describe the use of gene amplification, followed by endonuclease digestion and agarose gel electrophoresis, to discern size and restriction site differences. LPB5, a common allele associated with reduced low density lipoprotein clearance and hypercholesterolaemia in pigs, is...
Topics
- Alleles
- Animals
- Apolipoproteins B
- Base Sequence
- Electrophoresis, Agar Gel
- Molecular Sequence Data
- Oligonucleotides
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Swine