Article
ELISA detection of restriction site polymorphisms in the pig ryanodine receptor locus.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Aug 1998
Truett G E, Walker J A, Wilson J B, Redmann S M, Tulley R T, Eckardt G R, Plastow G, Lefevre M
Abstract excerpt
We compare two strategies for ELISA detection of restriction site polymorphisms (EDRSP) that are suitable for high-throughput genotyping of the pig ryanodine receptor point mutation (RYR1(hal)). In both procedures, target DNA is amplified by PCR with one primer that is 5' biotinylated and a secon...
Topics
- Animals
- DNA Primers
- DNA Restriction Enzymes
- Enzyme-Linked Immunosorbent Assay
- Genotype
- Point Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Ryanodine Receptor Calcium Release Channel
- Swine