Article
Molecular localization of ion selectivity sites within the pore of a human L-type cardiac calcium channel.
The Journal of biological chemistry - 25 Jun 1993
Tang S, Mikala G, Bahinski A, Yatani A, Varadi G, Schwartz A
Abstract excerpt
A highly conserved position of negatively charged amino acids is present in the SS2 segments of the S5-S6 linker regions among calcium channels. We report here that replacing Glu residues at this position alters the ion selectivity of the human cardiac calcium channel. Substituting Glu334 in motif I or Glu1086 in motif III with Lys produced mutant calcium channels that permeated sodium ions 10-fold more...
Topics
- Amino Acid Sequence
- Binding Sites
- Biological Transport
- Calcium Channels
- Cations
- Electrophysiology
- Glutamates
- Glutamic Acid
- Humans
- Molecular Sequence Data
- Mutation