Article
Propionate metabolism in cultured human cells after overexpression of recombinant methylmalonyl CoA mutase: implications for somatic gene therapy.
Somatic cell and molecular genetics - 1 Nov 1992
Wilkemeyer M, Stankovics J, Foy T, Ledley F D
Abstract excerpt
Strategies for somatic gene therapy must consider the metabolic consequences of expressing the recombinant gene product in addition to methods for gene transfer and expression. We describe studies of propionate metabolism in cultured cells transfected with methylmalonyl CoA mutase (MCM), the enzy...
Topics
- Amino Acid Metabolism, Inborn Errors
- Carcinoma, Hepatocellular
- Cells, Cultured
- Fibroblasts
- Genetic Therapy
- Humans
- Methylmalonyl-CoA Mutase
- Mutation
- Propionates
- Recombinant Proteins
- Transfection
- Tumor Cells, Cultured