Article
A rapid colorimetric in situ mRNA hybridization technique using hyperbiotinylated oligonucleotide probes for analysis of mdr1 in mouse colon carcinoma cells.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society - 1 Apr 1993
Bucana C D, Radinsky R, Dong Z, Sanchez R, Brigati D J, Fidler I J
Abstract excerpt
We describe the development of a rapid colorimetric in situ hybridization technique utilizing oligonucleotide probes labeled with six biotin molecules at the 3' end to detect mdr1 in mouse colon cancer cells growing in culture and in vivo. mRNA integrity was verified by the use of a multibiotinyl...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 1
- Animals
- Antisense Elements (Genetics)
- Base Sequence
- Blotting, Northern
- Colonic Neoplasms
- Drug Resistance
- Frozen Sections
- In Situ Hybridization
- Male