Article
Effects of amino acid variations in recombinant Der fII on its human IgE and mouse IgG recognition.
International archives of allergy and immunology - 1 Sept 1994
Nishiyama C, Yuuki T, Usui Y, Iwamoto N, Okumura Y, Okudaira H
Abstract excerpt
Amino acid sequencing of the major mite allergen Der fII purified from mite body extract revealed that it is a mixture of at least two variants. Substitutions were found only at positions in which amino acid variations were predicted from the nucleotide sequences of three cloned cDNAs. When cDNAs corresponding to the three variants were expressed in Escherichia coli, Der fII proteins were produced as inclusion...
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