Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR.

doi:10.1016/0167-4781(94)90260-7

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Article

Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR.

Biochimica et biophysica acta - 13 Sept 1994

Holstege F C, van der Vliet P C, Timmers H T

Abstract excerpt

Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

Topics

Artifacts
Base Sequence
DNA, Complementary
DNA-Binding Proteins
Genetic Variation
Humans
Molecular Sequence Data
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
TATA-Box Binding Protein
Transcription Factors

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