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Article

Increasing the synthesis/hydrolysis ratio of aminoacylase 1 by site-directed mutagenesis.

Biochimie - 1 Jan 2010

Wardenga Rainer, Lindner Holger A, Hollmann Frank, Thum Oliver, Bornscheuer Uwe

Abstract excerpt

Aminoacylase-1 from pig kidney (pAcy1) catalyzes the highly stereoselective acylation of amino acids, a useful conversion for the preparation of optically pure N-acyl-l-amino acids. The kinetic of this thermodynamically controlled conversion is determined by maximal velocities for synthesis (V(mS)) and hydrolysis (V(mH)) of the N-acyl-l-amino acid. To investigate which parameter affects maximal velocities, we...

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Identifiers

PMID: 19818829
DOI: 10.1016/j.biochi.2009.09.017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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