Article
Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells.
Blood - 15 Sept 1994
Maxwell P H, Ferguson D J, Osmond M K, Pugh C W, Heryet A, Doe B G, Johnson M H, Ratcliffe P J
Abstract excerpt
We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has...
Topics
- Anemia
- Animals
- Antigens, Polyomavirus Transforming
- Erythropoietin
- Gene Expression
- Hypoxia
- Immunohistochemistry
- Liver
- Mice
- Mice, Transgenic
- Microscopy, Immunoelectron
- Organ Specificity
- Phenotype
- RNA, Messenger
- Recombinant Fusion Proteins