Article
Mutation in the hydrophobic domain of ATP synthase subunit 4 (subunit b) of yeast mitochondria disturbs coupling between proton translocation and catalysis.
Biochemistry - 16 Aug 1994
Razaka-Jolly D, Rigoulet M, Guérin B, Velours J
Abstract excerpt
We introduced mutations to test the function of the hydrophobic sector of subunit 4 from Saccharomyces cerevisiae ATP synthase. Mutations were introduced at the chromosomic locus by homologous transformation of a strain disrupted in the ATP4 gene. The strain carrying the replacement Leu68-Val69--...
Topics
- Base Sequence
- Biological Transport
- Catalysis
- DNA Mutational Analysis
- Dicyclohexylcarbodiimide
- Drug Resistance
- Genes, Fungal
- Mitochondria
- Molecular Sequence Data
- Mutation
- Oligomycins
- Oxidative Phosphorylation
- Phenotype
- Protein Conformation
- Proton-Translocating ATPases
- Protons
- Saccharomyces cerevisiae
- Structure-Activity Relationship