Article
The C-terminal region of subunit 4 (subunit b) is essential for assembly of the F0 portion of yeast mitochondrial ATP synthase.
European journal of biochemistry - 1 Apr 1992
Paul M F, Guerin B, Velours J
Abstract excerpt
The role of the C-terminal part of yeast ATP synthase subunit 4 (subunit b) in the assembly of the whole enzyme was studied by using nonsense mutants generated by site-directed mutagenesis. The removal of at least the last 10 amino-acid residues promoted mutants which were unable to grow with glycerol or lactate as carbon source. These mutants were devoid of subunit 4 and of another F0 subunit, the...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Base Sequence
- Culture Media
- Electrophoresis, Polyacrylamide Gel
- Mitochondria
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligomycins
- Oligonucleotides
