Article
Modulation at a distance of proton conductance through the Saccharomyces cerevisiae mitochondrial F1F0-ATP synthase by variants of the oligomycin sensitivity-conferring protein containing substitutions near the C-terminus.
Journal of bioenergetics and biomembranes - 1 Dec 2000
Boyle G M, Roucou X, Nagley P, Devenish R J, Prescott M
Abstract excerpt
We have sought to elucidate how the oligomycin sensitivity-conferring protein (OSCP) of the mitochondrial F(1)F(0)-ATP synthase (mtATPase) can influence proton channel function. Variants of OSCP, from the yeast Saccharomyces cerevisiae, having amino acid substitutions at a strictly conserved residue (Gly166) were expressed in place of normal OSCP. Cells expressing the OSCP variants were able to grow on...
Topics
- Adenosine Triphosphatases
- Amino Acid Substitution
- Base Sequence
- Carrier Proteins
- DNA Primers
- DNA, Fungal
- Enzyme Stability
- Genetic Variation
- Membrane Potential, Mitochondrial
- Membrane Proteins
- Mitochondrial Proton-Translocating ATPases
- Mutagenesis, Site-Directed