Article
Bioenergetic and structural consequences of allotopic expression of subunit 8 of yeast mitochondrial ATP synthase. The hydrophobic character of residues 23 and 24 is essential for maximal activity and structural stability of the enzyme complex.
European journal of biochemistry - 1 Apr 1999
Roucou X, Artika I M, Devenish R J, Nagley P
Abstract excerpt
Subunit 8 (Y8), a mitochondrially encoded subunit of the F0 sector of the F1F0-ATP synthase is essential for oxidative phosphorylation. We have previously introduced the technique of allotopic expression to study the structure/function of Y8, whereby an artificial Y8 gene is expressed in the nucleus of cells lacking a functional mitochondrial Y8, thus generating assembly of a functional F1F0-ATPase complex. In...
Topics
- Adenosine Triphosphate
- Enzyme Stability
- Fluorescence
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Kinetics
- Mitochondria
- Oligomycins
- Oxidative Phosphorylation
- Phenotype
- Proton-Translocating ATPases
- Rhodamine 123