Article
Murine osteoclasts and spleen cell polykaryons are distinguished by mRNA phenotyping.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Apr 1994
Tong H S, Sakai D D, Sims S M, Dixon S J, Yamin M, Goldring S R, Snead M L, Minkin C
Abstract excerpt
To probe osteoclast gene expression, we combined the techniques of cell microisolation and RT-PCR to develop a novel and sensitive method for the isolation and mRNA phenotyping of small numbers of authentic osteoclasts and spleen cell polykaryons. Using this method we report (1) direct evidence f...
Topics
- Animals
- Base Sequence
- Cell Separation
- DNA Primers
- Gene Expression
- Hematopoietic Stem Cells
- Macrophages
- Mice
- Molecular Sequence Data
- Osteoclasts
- Phenotype
- Polymerase Chain Reaction
- RNA, Messenger
- Spleen