Article
Isolation and gene expression of haematopoietic-cell-free preparations of highly purified murine osteocytes.
Bone - 1 Mar 2015
Chia Ling Yeong, Walsh Nicole C, Martin T John, Sims Natalie A
Abstract excerpt
To define their gene expression and function, osteocytes are commonly isolated and purified by fluorescence-activated cell sorting (FACS) from mice expressing GFP directed by the dentin matrix protein 1 (Dmp1) promoter (DMP1-GFP). These cells express mRNA for osteocyte genes, including sclerostin (Sost) and Dmp1, and genes associated with the osteoclast phenotype: Dcstamp, Oscar, Cathepsin K (Ctsk), tartrate...
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