Article
Mutations affecting a regulated, membrane-associated esterase in Salmonella typhimurium LT2.
Molecular & general genetics : MGG - 15 Jun 1994
Collin-Osdoby P, Miller C G
Abstract excerpt
Mutations at the apeA locus in Salmonella typhimurium lead to loss of a soluble enzyme ("protease I") that hydrolyzes the chromogenic endoprotease substrate N-acetyl phenylalanine beta-naphthyl ester. We have isolated pseudorevertants of S. typhimurium apeA mutations that have regained the ability to hydrolyze this compound. These pseudorevertants contain mutations (apeR) that lead to overproduction of a...
Topics
- Esterases
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Membrane Proteins
- Mutation
- Phenylalanine
- Salmonella typhimurium
- Transcription, Genetic