Article
Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.
Journal of bacteriology - 1 Feb 1993
Ichihara S, Matsubara Y, Kato C, Akasaka K, Mizushima S
Abstract excerpt
Clones carrying the gene encoding a proteinase were isolated from Clarke and Carbon's collection, using a chromogenic substrate, N-benzyloxycarbonyl-L-phenylalanine beta-naphthyl ester. The three clones isolated, pLC6-33, pLC13-1, and pLC36-46, shared the same chromosomal DNA region. A 0.9-kb Sau...
Topics
- Amino Acid Sequence
- Base Sequence
- Chromosome Mapping
- Cloning, Molecular
- DNA Mutational Analysis
- DNA, Bacterial
- Endopeptidases
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Restriction Mapping
- Sequence Deletion