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Article

Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.

Journal of bacteriology - 1 Feb 1993

Ichihara S, Matsubara Y, Kato C, Akasaka K, Mizushima S

Abstract excerpt

Clones carrying the gene encoding a proteinase were isolated from Clarke and Carbon's collection, using a chromogenic substrate, N-benzyloxycarbonyl-L-phenylalanine beta-naphthyl ester. The three clones isolated, pLC6-33, pLC13-1, and pLC36-46, shared the same chromosomal DNA region. A 0.9-kb Sau3AI fragment within this region was found to be responsible for the overproduction of the proteinase, and the...

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Identifiers

PMID: 8432696
DOI: 10.1128/jb.175.4.1032-1037.1993
PMCID: PMC193016

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