Article
Isolation and characterization of a Tn5-induced tolQ mutant of Escherichia coli.
Canadian journal of microbiology - 1 Jun 1994
Madrid C, Badenas C, Juárez A
Abstract excerpt
Transposon mutagenesis was used to isolate an Escherichia coli mutant that released into the external medium a heterologous protein, the Aeromonas hydrophila aerolysin. Genetic mapping and phenotypic characterization of E. coli CM209 showed that Tn5 is inserted in the tolQ gene. This mutant strai...
Topics
- Aeromonas hydrophila
- Bacterial Proteins
- Bacterial Toxins
- Base Sequence
- Cell Fractionation
- Chromosome Mapping
- Colicins
- DNA Transposable Elements
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Hemolysin Proteins
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Phenotype
- Pore Forming Cytotoxic Proteins