Article
Adaptation of Pseudomonas sp. GJ1 to 2-bromoethanol caused by overexpression of an NAD-dependent aldehyde dehydrogenase with low affinity for halogenated aldehydes.
Archives of microbiology - 1 Apr 1996
Van der Ploeg J R, Kingma J, De Vries E J, Van der Ven J G, Janssen D B
Abstract excerpt
Pseudomonas sp. GJ1 is able to grow with 2-chloroethanol as the sole carbon and energy source, but not with 2-bromoethanol, which is toxic at low concentrations (1 mM). A mutant that could grow on 2-bromoethanol with a growth rate of 0.034 h-1 at concentrations up to 5 mM was isolated and designa...
Topics
- Acetaldehyde
- Adaptation, Physiological
- Alcaligenes
- Aldehyde Dehydrogenase
- Aldehydes
- Amino Acid Sequence
- Enzyme Inhibitors
- Ethanol
- Gene Expression
- Kinetics
- Molecular Sequence Data
- Molecular Weight
- Mutation
- Protein Conformation
- Pseudomonas
- Sequence Homology, Amino Acid
- Species Specificity
- Vibrio cholerae