Combined immunocytochemistry and fluorescence in situ hybridization for simultaneous tricolor detection of cell cycle, genomic, and phenotypic parameters of tumor cells.

Abstract excerpt

We describe the development and application of a sensitive high-resolution fluorescence alkaline phosphatase (APase)-Fast Red immunocytochemical (ICC) staining method in combination with fluorescence in situ hybridization (ISH) and bromodeoxyuridine (BrdU) detection. The high fluorescence intensi...

Topics

• Alkaline Phosphatase
• Bromodeoxyuridine
• Cell Cycle
• DNA Probes
• DNA, Neoplasm
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Kinetics
• Microscopy, Fluorescence
• Neoplasm Proteins