One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation.

doi:10.1002/(SICI)1097-0320(19961201)25:4<358::AID-CYTO7>3.0.CO;2-9

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Article

One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation.

Cytometry - 1 Dec 1996

Corver W E, Bonsing B A, Abeln E C, Vlak-Theil P M, Cornelisse C J, Fleuren G J

Abstract excerpt

We have developed a "one-tube" triple staining procedure that allows the identification of intratumor phenotypic subpopulations by FCM. Solid tumors were dissociated by a combined mechanical/ enzymatic method. Ovarian ascites tumor cell aggregates were enzymatically dissociated using trypsin. An...

Topics

Biomarkers
Cell Survival
Coloring Agents
DNA, Neoplasm
Female
Flow Cytometry
Fluorescein-5-isothiocyanate
Genetic Heterogeneity
Humans
Intermediate Filaments
Lasers
Neoplasms

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Ovarian Neoplasms

Phenotype

Phycoerythrin

Ploidies

Proliferating Cell Nuclear Antigen

Propidium

Source

Open in PubMed

Identifiers

PMID: 8946143
DOI: 10.1002/(SICI)1097-0320(19961201)25:4<358::AID-CYTO7>3.0.CO;2-9

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8946143-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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