Article
One-tube triple staining method for flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation.
Cytometry - 1 Dec 1996
Corver W E, Bonsing B A, Abeln E C, Vlak-Theil P M, Cornelisse C J, Fleuren G J
Abstract excerpt
We have developed a "one-tube" triple staining procedure that allows the identification of intratumor phenotypic subpopulations by FCM. Solid tumors were dissociated by a combined mechanical/ enzymatic method. Ovarian ascites tumor cell aggregates were enzymatically dissociated using trypsin. An antikeratin 8/18 MAb was used to label the epithelial fraction of these tumor samples. A second MAb directed against...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.