[Precise excision of TN5 in Escherichia coli K12 mutants with alterations in common component of phosphotransferase system and in subunits of DNA-gyrase].

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Article

[Precise excision of TN5 in Escherichia coli K12 mutants with alterations in common component of phosphotransferase system and in subunits of DNA-gyrase].

Molekuliarnaia genetika, mikrobiologiia i virusologiia - 1 Jan 2010

Dobrynina O Iu, Umiarov A M, Markov A P, Bol'shakova T N

Abstract excerpt

The effect of ptsH and gyrA mutations on precise excision (PE) of transposon Tn5 was studied in Escherichia coli K12. The conjugative plasmid with Tn5 integrated in the tet gene of Tn10 was used as a model in experiments. It was shown that mutational damage of HPr, a common component of the bacterial PEP-dependent phosphotransferase system (PTS), increased the frequency of PE. The alteration of the subunit A of...

Topics

Bacterial Proteins
DNA Gyrase
DNA Transposable Elements
Escherichia coli K12
Mutation
Phosphoenolpyruvate Sugar Phosphotransferase System
Protein Subunits
Transposases

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Open in PubMed

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PMID: 20361665

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