Article
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
FEMS microbiology letters - 1 Nov 1994
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Pühler A
Abstract excerpt
Efficient electroporation of Escherichia coli with plasmid DNA isolated from Corynebacterium glutamicum depends on the use of Mcr-deficient E. coli strains. The transformation frequency increased nearly 800-fold when the Mcr-deficient E. coli DH5 alpha MCR was used instead of E. coli DH5 alpha. We used E. coli strains with different mutations in the methyl-specific restriction systems to show that...
Topics
- Cloning, Molecular
- Corynebacterium
- DNA Restriction Enzymes
- DNA, Bacterial
- Escherichia coli
- Genetic Vectors
- Methylation
- Mutation
- Transformation, Bacterial