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Article

Manipulation of Corynebacterium glutamicum by gene disruption and replacement.

Bio/technology (Nature Publishing Company) - 1 Jan 1991

Schwarzer A, Pühler A

Abstract excerpt

We have developed a system for the genetic manipulation of the amino acid-producing Corynebacterium glutamicum. Gene disruption and replacement were achieved by introducing, via conjugation, Escherichia coli vector plasmids carrying manipulated C. glutamicum DNA fragments. We obtained stable mutants in which the chromosomal lysA gene, encoding meso-diaminopimelate decarboxylase, was interrupted by a...

Topics

Chromosome Deletion
Chromosomes
Cloning, Molecular
Conjugation, Genetic
Corynebacterium
DNA
Escherichia coli
Gene Expression Regulation
Mutagenesis, Insertional
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 1367217
DOI: 10.1038/nbt0191-84

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1367217-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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