In vitro maturation of prehead-like bacteriophage T4 polyheads: structural changes accompanying proteolytic cleavage and lattice expansion.

Abstract excerpt

We have studied cleavage and expansion of the major T4 phage capsid protein gp23 (56 kDa) using polyheads, an aberrant, polymorphic tubular variant of bacteriophage T4D, as a model system. In a first step, we have cleaved the 65-amino-acid-long amino-terminal "delta piece" by limited proteolysis with Staphylococcus aureus V8 protease (type XVII) at exactly the same position, i.e., between residues 65 and 66, at...

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