Mutational analysis of the murine AIDS-defective viral genome reveals a high reversion rate in vivo and a requirement for an intact Pr60gag protein for efficient induction of disease.

Abstract excerpt

Pr60gag appears to be the only protein encoded by the murine AIDS (MAIDS)-defective virus. To study the role of Pr60gag or some other sequences of the viral genome in the pathogenicity of the virus, we have generated mutants of the defective viral genome. These mutant defective viruses, prepared as helper-free stocks, were inoculated into susceptible C57BL/6 mice. Mutant Du5H-A virus, which had a stop codon...

Topics

• Animals
• Base Sequence
• Cells, Cultured
• DNA Primers
• Fibroblasts
• Gene Products, gag
• Genome, Viral
• Leukemia Virus, Murine
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data