Article
A stable hammerhead structure is not required for endonucleolytic activity of a ribozyme in vivo.
Gene - 4 Nov 1994
Steinecke P, Steger G, Schreier P H
Abstract excerpt
Cleavage of a specific target, the mRNA encoding the bacterial neomycin phosphotransferase, by mutant satellite RNA of subterranean clover mottle virus (sSCMoV) ribozymes (Rz) was used to study the role of the hammerhead (Hh) structure in Rz activity in cis and in trans. The bimolecular Rz-target...
Topics
- Amino Acid Sequence
- Base Sequence
- Computer Simulation
- Kanamycin Kinase
- Molecular Sequence Data
- Mosaic Viruses
- Mutation
- Nucleic Acid Conformation
- Phosphotransferases (Alcohol Group Acceptor)
- Plants, Toxic
- Plasmids
- Protoplasts
- RNA, Catalytic
- RNA, Messenger
- Structure-Activity Relationship
- Nicotiana
- Transfection