Article

Log-order improved <i>in trans</i> hammerhead ribozyme turnover rates: reevaluating therapeutic space for small catalytic RNAs

2022-04-26

Abstract excerpt

<h4>ABSTRACT</h4> We discovered an enhanced functionality hammerhead ribozyme ( EhhRz ), designed to act in trans against human rod opsin ( RHO ) mRNA, with turnover activity >300 nM min −1 under substrate-excess conditions and physiological Mg 2+ levels (1 mM). We developed a real-time moderate-throughput fluorescence quantitative hhRz kinetic assay, which is linear with substrate and product moles and supp...

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Literature Corpus work: 0088e4e1-6304-51eb-b999-f115234837cf
DOI: 10.1101/2022.04.26.489422

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