U6 snRNA genes of Arabidopsis are transcribed by RNA polymerase III but contain the same two upstream promoter elements as RNA polymerase II-transcribed U-snRNA genes.

Abstract excerpt

Previously we have demonstrated that the U2 snRNA genes from the higher plant Arabidopsis thaliana contain two upstream elements, the USE with sequence RTCCCACATCG and a -30 'TATA' box, which are essential for transcription by RNA polymerase II, and that the conserved spacing of about four helical DNA turns between these elements is important for optimal promoter function. We have now isolated three genes...

Topics

• Amanitins
• Base Sequence
• Blotting, Southern
• DNA-Directed RNA Polymerases
• Genes, Plant
• Molecular Sequence Data
• Mutation
• Plants
• Plants, Toxic
• Promoter Regions, Genetic
• Protoplasts