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Article

Structural details of Asp(B9) human insulin at low pH from two-dimensional NMR titration studies.

Biochemistry - 22 Nov 1994

Sørensen M D, Led J J

Abstract excerpt

A titration study of the dimeric Asp(B9) mutant of human insulin was performed using two-dimensional NMR spectroscopy. Based on 10 NOESY spectra recorded in the pH range 1.73-3.93, the pKa values of the seven carboxyl groups in the mutant were determined, and the titration shifts of 46 pH-dependent protons in non-ionizable groups were investigated. Further, the pKa values of the two histidine imidazole rings were...

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Source

Identifiers

PMID: 7947783
DOI: 10.1021/bi00250a025

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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