Structural details of Asp(B9) human insulin at low pH from two-dimensional NMR titration studies.

doi:10.1021/bi00250a025

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Article

Structural details of Asp(B9) human insulin at low pH from two-dimensional NMR titration studies.

Biochemistry - 22 Nov 1994

Sørensen M D, Led J J

Abstract excerpt

A titration study of the dimeric Asp(B9) mutant of human insulin was performed using two-dimensional NMR spectroscopy. Based on 10 NOESY spectra recorded in the pH range 1.73-3.93, the pKa values of the seven carboxyl groups in the mutant were determined, and the titration shifts of 46 pH-depende...

Topics

Amino Acid Sequence
Aspartic Acid
Electrochemistry
Humans
Hydrogen Bonding
Hydrogen-Ion Concentration
Insulin
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Mutation
Threonine
Valine

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