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Article

Altered ionization of the B13 Glu in insulin B9 and B10 mutants: a computational analysis.

Protein engineering, design & selection : PEDS - 1 Jul 2004

Greaves Richard B, Dodson Guy G, Verma Chandra S

Abstract excerpt

An experimentally determined pK(a) change of +2.50 units has been reported for the B13 Glu residue in a dimeric B9 Ser --> Asp insulin mutant relative to the native dimer. Poisson-Boltzmann electrostatics-based pK(a) calculations were performed to probe the effect of the B9 Ser --> Asp and B10 His --> Asp mutations on aggregation and the ionization behaviour of the B13 carboxylate. The method produced shifts of...

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Identifiers

PMID: 15326283
DOI: 10.1093/protein/gzh066

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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