Sequence-specific 1H-NMR assignments for the aromatic region of several biologically active, monomeric insulins including native human insulin.

doi:10.1016/0167-4889(90)90027-b

Back to search

Article

Sequence-specific 1H-NMR assignments for the aromatic region of several biologically active, monomeric insulins including native human insulin.

Biochimica et biophysica acta - 12 Jun 1990

Roy M, Lee R W, Kaarsholm N C, Thøgersen H, Brange J, Dunn M F

Abstract excerpt

The aromatic region of the 1H-FT-NMR spectrum of the biologically fully-potent, monomeric human insulin mutant, B9 Ser----Asp, B27 Thr----Glu has been investigated in D2O. At 1 to 5 mM concentrations, this mutant insulin is monomeric above pH 7.5. Coupling and amino acid classification of all aro...

Topics

Amino Acid Sequence
Chemical Phenomena
Chemistry
Crystallization
Humans
Hydrogen-Ion Concentration
Insulin
Magnetic Resonance Spectroscopy
Mutation
Protein Conformation

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.