Article
Determination of ABO genotypes with DNA extracted from formalin-fixed, paraffin-embedded tissues.
International journal of legal medicine - 1 Jan 1994
Yamada M, Yamamoto Y, Tanegashima A, Kane M, Ikehara Y, Fukunaga T, Nishi K
Abstract excerpt
The gene encoding the specific glycosyltransferases which catalyze the conversion of the H antigen to A or B antigens shows a slight but distinct variation in its allelic nucleotide sequence and can be divided into 6 genotypes when digested with specific restriction enzymes. We extracted DNA from formalin-fixed, paraffin-embedded tissues using SDS/proteinase K treatment followed by phenol/chloroform extraction....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
