Article
Determination of ABO glycosyltransferase genotypes by use of polymerase chain reaction and restriction enzymes.
Transfusion - 1 Mar 1995
Stroncek D F, Konz R, Clay M E, Houchins J P, McCullough J
Abstract excerpt
BACKGROUND: The molecular basis of red cell ABO group antigens has been determined. The genes encoding the group A and B glycosyltransferases and a nonfunctional group O transferase have been cloned and sequenced. All three genes were similar. When compared to the nucleotide sequence of the A gene, the O gene has a one-base deletion that leads to a frame shift and results in a nonfunctional protein. The B gene...
Topics
- ABO Blood-Group System
- Alleles
- Base Sequence
- DNA
- DNA Restriction Enzymes
- Galactosyltransferases
- Genotype
- Humans
- Leukocytes
- Molecular Sequence Data
- Pedigree