Article
Gene regulation by temperature-sensitive p53 mutants: identification of p53 response genes.
Proceedings of the National Academy of Sciences of the United States of America - 25 Oct 1994
Buckbinder L, Talbott R, Seizinger B R, Kley N
Abstract excerpt
The ability of the p53 protein to act as a sequence-specific transcriptional activator suggests that genes induced by p53 may encode critical mediators of p53 tumor suppression. Using a tetracycline-regulated p53 expression system and cDNA library subtraction procedure, we identified several p53-induced gene transcripts in human Saos-2 osteosarcoma cells that are novel on the basis of their size, regulation, and...
Topics
- Apoptosis
- Base Sequence
- Binding Sites
- Blotting, Northern
- Blotting, Western
- Cell Line
- DNA, Neoplasm
- Gene Expression Regulation, Neoplastic
- Gene Library
- Genes, p53
- Humans