Article
Conditional lethality of a yeast strain expressing human RHOA in place of RHO1.
Proceedings of the National Academy of Sciences of the United States of America - 27 Sept 1994
Qadota H, Anraku Y, Botstein D, Ohya Y
Abstract excerpt
The yeast RHO1 GTPase, which has 72% amino acid sequence identity with its human counterpart, RHOA, is essential for growth, although the reason has not been investigated. We report here that yeast strains that rely solely on expression of human RHOA in place of RHO1 are able to grow at 23 degree...
Topics
- Adenosine Diphosphate Ribose
- Amino Acid Sequence
- Consensus Sequence
- Fungal Proteins
- GTP-Binding Proteins
- Genes, Lethal
- Genetic Complementation Test
- Genotype
- Humans
- Molecular Sequence Data
- Plasmids
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Sequence Homology, Amino Acid
- rho GTP-Binding Proteins
- rhoA GTP-Binding Protein