Article
The Ras/Raf signaling pathway is required for progression of mouse embryos through the two-cell stage.
Molecular and cellular biology - 1 Oct 1994
Yamauchi N, Kiessling A A, Cooper G M
Abstract excerpt
We have used microinjection of antisense oligonucleotides, monoclonal antibody, and the dominant negative Ras N-17 mutant to interfere with Ras expression and function in mouse oocytes and early embryos. Microinjection of either ras antisense oligonucleotides or anti-Ras monoclonal antibody Y13-259 did not affect normal progression of oocytes through meiosis and arrest at metaphase II. However, microinjection of...
Topics
- Animals
- Antibodies, Monoclonal
- Base Sequence
- Blastocyst
- Cell Division
- Cleavage Stage, Ovum
- Gene Expression Regulation
- Meiosis
- Mice
- Molecular Sequence Data
- Morula