Article
Selection of cold-adapted mutants of human rotaviruses that exhibit various degrees of growth restriction in vitro.
Journal of virology - 1 Nov 1994
Hoshino Y, Kapikian A Z, Chanock R M
Abstract excerpt
Group A human rotavirus strains D, Wa, DS-1, and P were originally recovered from children with diarrhea. In an attempt to attenuate virulent, wild-type human rotaviruses of major epidemiological importance for use in a live oral vaccine, two reference rotavirus strains, D and DS-1, and two laboratory-generated reassortants, Wa x DS-1 and Wa x P, were subjected to cold adaptation. Collectively, these viruses...
Topics
- Animals
- Cells, Cultured
- Child
- Chlorocebus aethiops
- Cold Temperature
- Humans
- Mutation
- Rotavirus
- Viral Vaccines