Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Genomic changes detected after serial passages in cell culture of virulent human G1P[8] rotaviruses.

Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases - 1 Nov 2016

Tsugawa Takeshi, Tsutsumi Hiroyuki

Abstract excerpt

Serial passages of a virulent mouse rotavirus in cell cultures caused a loss of virulence in mice. To gain insight into the genomic mutations in human rotavirus during cell culture and its attenuation in humans, we serially passaged three wild type human G1P[8] rotavirus strains (Wa, DC3695, DC5685) derived from diarrheal stool samples up to 60 times in two different cell cultures (human colon adenocarcinoma cell...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Chlorocebus aethiops
Share to Genome, Viral

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to HT29 Cells

Share to Rotavirus

Share to Rotavirus Infections

Share to Serial Passage

Share to Vero Cells

Share to Virulence

Source

Identifiers

PMID: 27543393
DOI: 10.1016/j.meegid.2016.08.015

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Genomic changes detected after serial passages in cell culture of virulent human G1P[8] rotaviruses.PMID 27543393Read ↗

Human Rotaviruses of Multiple Genotypes Acquire Conserved VP4 Mutations during Serial Passage.Semantic proximity Read ↗

Alterations in the sequence of the gene 4 from a human rotavirus after multiple passages in HepG2 liver cells.Semantic proximity Read ↗

Mutations of two amino acids in VP5 mediate the attenuation of human rotavirus vaccine: evidence from in vitro and in vivo studies.Semantic proximity Read ↗

Amino Acid Substitutions in Positions 385 and 393 of the Hydrophobic Region of VP4 May Be Associated with Rotavirus Attenuation and Cell Culture Adaptation.Semantic proximity Read ↗

Identification of Murine Rotavirus Virulence Determinants Using Bidirectional Selective Passaging and a Reverse Genetics System.Semantic proximity Read ↗

VP4 Mutation Boosts Replication of Recombinant Human/Simian Rotavirus in Cell Culture.Semantic proximity Read ↗

Whole Genome Sequences of the Wildtype AU-1 Rotavirus A Strain: The Prototype of the AU-1-like Genotype Constellation.Semantic proximity Read ↗

Sequence analysis of VP4 genes of wild type and culture adapted human rotavirus G1P[8] strains.Semantic proximity Read ↗

Reverse Genetics Approach for Developing Rotavirus Vaccine Candidates Carrying VP4 and VP7 Genes Cloned from Clinical Isolates of Human Rotavirus.Semantic proximity Read ↗

Generation of Simian Rotavirus Reassortants with VP4- and VP7-Encoding Genome Segments from Human Strains Circulating in Africa Using Reverse Genetics.Semantic proximity Read ↗

Reverse Genetics System for a Human Group A Rotavirus.Semantic proximity Read ↗

Reverse Genetics of Murine Rotavirus: A Comparative Analysis of the Wild-Type and Cell-Culture-Adapted Murine Rotavirus VP4 in Replication and Virulence in Neonatal MiceSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.