Article
Exploiting spore-autonomous fluorescent protein expression to quantify meiotic chromosome behaviors in Saccharomyces cerevisiae.
Genetics - 1 Oct 2011
Thacker Drew, Lam Isabel, Knop Michael, Keeney Scott
Abstract excerpt
The budding yeast Saccharomyces cerevisiae has proven to be a rich source of information about the mechanisms and regulation of homologous recombination during meiosis. A common technique for studying this process involves microdissecting the four products (ascospores) of a single meiosis and analyzing the configuration of genetic markers in the spores that are viable. Although this type of analysis is powerful,...
Topics
- Chromosome Segregation
- Chromosomes, Fungal
- Crossing Over, Genetic
- Flow Cytometry
- Homologous Recombination
- Luminescent Proteins
- Meiosis
- Microscopy, Fluorescence
- Mutation
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Spores, Fungal
- Time Factors